{"id":2847,"date":"2023-04-27T15:52:34","date_gmt":"2023-04-27T15:52:34","guid":{"rendered":"http:\/\/qfm.uc.pt\/?page_id=2847"},"modified":"2024-05-02T14:36:44","modified_gmt":"2024-05-02T14:36:44","slug":"pinewallen","status":"publish","type":"page","link":"https:\/\/qfm.uc.pt\/index.html\/language\/en\/projects\/pinewallen\/","title":{"rendered":"Linking pine cell wall composition and structure to pinewood nematode resistance under climate change – PineWALL"},"content":{"rendered":"\n

Pine-related goods and activities make up a substantial share of the Portuguese forestry sector, and more generally, of the Portuguese Economy. Therefore, it is vital for this resource to be protected, especially as uncertain economic times lie ahead. The pinewood nematode (PWN), Bursaphelenchus xylophilus<\/em>, is a major threat to this sector, as it causes the pine wilt disease (PWD), leading to high losses in conifer forests. The PWN infects various Pinus<\/em> spp., namely the maritime pine (P. pinaster<\/em>), which is widely distributed in Portugal and is one of the most susceptible species. Other Pinus<\/em> spp., such as the stone (P. pinea<\/em>) and Aleppo (P. halepensis<\/em>) pines are less, or even non-susceptible to the PWN. However, the reasons for these differences in susceptibility to PWN are not well understood. In recent years, members of our research team have independently worked on furthering our knowledge about PWN pathogenicity mechanisms and on host defence mechanisms. With this preliminary database at our disposal, we have now conceived the PineWALL project. This will be an innovative transdisciplinary approach focused on an often-overlooked aspect, which is how the pine cell wall (CW) composition and structure can influence the host susceptibility\/resistance to PWN infection under a climate change scenario. The PWN uses its stylet to mechanically breach host CW, while releasing hydrolytic enzymes against all groups of plant CW glycans. These enzymes act by softening the CW, facilitating PWN migration within the plant. Nonetheless, there is a lack of studies focused on CW characterisation in function of PWN infection. With PineWALL we aim at addressing this gap in our knowledge. This project will focus on how Pinus<\/em> spp. traits are linked to PWN susceptibility\/resistance. Our main premise is that CW composition and structure are associated to varying degrees of susceptibility to PWN. Moreover, as drier and warmer conditions are likely to exacerbate vulnerability to PWN, we need to better understand the effects of varying environmental conditions on host susceptibility\/resistance to PWN infection. With this in mind, we have defined seven interconnected tasks that will make up the workplan for PineWALL. Susceptible and non-susceptible pine species (P. pinaster<\/em>, P. pinea<\/em>, P. halepensis<\/em>) and P. pinaster<\/em> genotypes with different susceptibilities to PWN will be inoculated with the nematodes and disease progression will be monitored while plants are grown under controlled temperatures and soil water contents. The morphology\/anatomy of susceptible and less susceptible Pinus<\/em> spp. will be compared, in order to understand whether anatomical features have implications on disease progression. High-throughput vibrational spectroscopy methods will be used for an overall characterisation of the Pinus<\/em> spp. CW. Chemical analytical methods will be used to perform a targeted characterisation of different CW fractions. Moreover, two immunological approaches will be employed to unveil the glycome profile of Pinus<\/em> spp. CW, and to reveal in muro<\/em> glycan distributions by in situ immunolabelling. Thus, helping to understand how CW glycans are linked to PWN susceptibility\/resistance. Data modelling will be performed to integrate all collected data, allowing the identification of biomarkers which would be used to predict PWN susceptibility\/resistance, ultimately contributing to the modelling of a P. pinaster<\/em> ideotype, better adapted to changing climatic and phytopathological conditions. PineWALL will highly benefit from the Research and Higher Education platforms at the Universities of Coimbra and Porto, which are the two main institutions of the project. Furthermore, the multidisciplinary nature of PineWALL is the result of careful planning to include diverse but complementary skills within the team. Thus, each team member will uniquely contribute to the project, while relying on the skills of others, towards a shared purpose. This purpose being of producing a comprehensive picture of Pinus<\/em> spp. CW in the context of PWN infection under a changing climate scenario. These studies will provide a wider understanding of PWN infection and host defence responses, and will be a valuable resource for researchers working with this detrimental plant pathogen. Lastly, as PineWALL aims at having a positive impact on society, we will interact directly with stakeholders in the forestry sector, promoting joint discussions to find solutions for problems affecting the Portuguese pine sector.<\/p>\n

State<\/b>
Ongoing
Period<\/b>
2021 to 2024
Funding<\/b>
239.781,74 \u20ac
Project code<\/b>
PTDC\/ASP-SIL\/3142\/2020
Funding entity<\/b>
Portuguese Foundation for Science and Technology (FCT)<\/p>\n

Members<\/b>
Ricardo da Costa<\/a> (Investigador Respons\u00e1vel)
Lu\u00eds Fonseca<\/a>
Hugo Silva<\/a>
Filipe Campelo<\/a>
Carmen Diniz<\/a>
Isabel Abrantes<\/a>
Joana Marques<\/a>
Joana Cardoso<\/a>
Jo\u00e3o Martins
Jorge Canhoto<\/a>
Lu\u00eds Batista de Carvalho<\/a>
Maria Paula Marques<\/a>
Maria Jo\u00e3o Ferreira<\/a>
S\u00edlvia Coimbra<\/a><\/p>\n

Project Dissemination<\/b><\/p>\n

Radio Antena 1 (Portugal), Programme “Portugal em Direto”<\/a> (from minute 19).<\/p>\n

Radio Regional do Centro (Portugal), Programme “Regional Rural”.<\/a><\/p>\n

Event website. “A Floresta Portuguesa num Mundo em Mudan\u00e7a”<\/a><\/p>\n

Magazine article (Portugal): Gazeta Rural n\u00ba 451, pp. 36-37.<\/a><\/p>\n

Newspaper article (Portugal): Di\u00e1rio as Beiras; “A Floresta Portuguesa num Mundo em Mudan\u00e7a”.<\/a><\/p>\n

Online News article (Portugal): Ind\u00fastria e Ambiente; FCTUC realiza evento sobre “A Floresta Portuguesa num Mundo em Mudan\u00e7a”.<\/a><\/p>\n

Online News article (Portugal): Not\u00edcias UC; Universidade de Coimbra promove encontro sobre “A Floresta Portuguesa num Mundo em Mudan\u00e7a”.<\/a><\/p>\n

Online News article (Portugal): Agroportal; “A Floresta Portuguesa num Mundo em Mudan\u00e7a – 23 de abril – Coimbra”.<\/a><\/p>\n

Dissemination of the project at the European Researchers\u2019 Night 2022\/2023 “Noite Europeia dos Investigadores”<\/a> \u2013 Hands-on activities and project leaflet<\/a> distribution to public (Portugal).<\/p>\n

Dissemination of the project at the “Dia Internacional da Floresta” 21.03 DCV \u2013 Hands-on activities and project leaflet distribution to public (Portugal).<\/a><\/p>\n

Radio Antena 1 (Portugal), Programme \u201c90 Segundos de Ci\u00eancia\u201d.<\/a><\/p>\n

Radio Canal Extremadura (Spain), Programme “La Mirada Verde”.<\/a><\/p>\n

Dissemination of the project in the European Researchers’ Night 2021\/2022 \u201cNoite Europeia dos Investigadores\u201d \u2013 Hands-on activities and project leaflet distribution to public (Portugal).<\/a><\/p>\n

Magazine article (Portugal): Gazeta Rural n\u00ba 376, pp. 26-27.<\/a><\/p>\n

Newspaper article (Spain): Agencia EFE; La pared celular de los pinos, soluci\u00f3n para acabar con la plaga de nem\u00e1todo.<\/a><\/p>\n

Newspaper article (Portugal): Di\u00e1rio as Beiras; Estudo quer apurar por que raz\u00e3o uns pinheiros resistem ao nem\u00e1todo e outros n\u00e3o.<\/a><\/p>\n

Newspaper article (Portugal): Di\u00e1rio de Coimbra; Porque \u00e9 que h\u00e1 uns pinheiros que resistem ao nem\u00e1todo e outros n\u00e3o?<\/a><\/p>\n

Online News article (Argentina): Clar\u00edn EFE; La pared celular de los pinos, soluci\u00f3n para acabar con la plaga de nematodo.<\/a><\/p>\n

Online News article (Latin America): InfoBae; La pared celular de los pinos, soluci\u00f3n para acabar con la plaga de nematodo.<\/a><\/p>\n

Online News article (Latin America): HolaNews; La pared celular de los pinos, soluci\u00f3n para acabar con la plaga de nematodo.<\/a><\/p>\n

Online News article (Spain): La Vanguardia; La pared celular de los pinos, soluci\u00f3n para acabar con la plaga de nematodo.<\/a><\/p>\n

Online News article (Spain): elDiario.es; La pared celular de los pinos, soluci\u00f3n para acabar con la plaga de nematodo.<\/a><\/p>\n

Online News article (Venezuela): El Siglo; La pared celular de los pinos, soluci\u00f3n para acabar con la plaga de nematodo.<\/a><\/p>\n

Online News article (USA): La Conexion USA; La pared celular de los pinos, soluci\u00f3n para acabar con la plaga de nematodo.<\/a><\/p>\n

Online News article (Bolivia): Los Tiempos; La pared celular de los pinos, soluci\u00f3n para acabar con la plaga de nematodo.<\/a><\/p>\n

Online News article (Argentina): TN news; La pared celular de los pinos, soluci\u00f3n para acabar con la plaga de nematodo.<\/a><\/p>\n

Online News article (Portugal): Agricultura & Mar; Investigadores da Universidade de Coimbra estudam combate \u00e0 doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): Agroportal; Projeto PineWALL prop\u00f5e estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): Agroportal; Estudo quer apurar por que raz\u00e3o uns pinheiros resistem ao nem\u00e1todo e outros n\u00e3o.<\/a><\/p>\n

Online News article (Portugal): Ambiente Magazine; Projeto da Universidade de Coimbra prop\u00f5e estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Entry in Blog: Ba\u00eda da Lusofonia; Projeto PineWALL prop\u00f5e estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): BeiraNews; Projeto PineWALL prop\u00f5e estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): CONFAGRI; Estudo quer apurar por que raz\u00e3o uns pinheiros resistem ao nem\u00e1todo e outros n\u00e3o.<\/a><\/p>\n

Online News article (Portugal): Campe\u00e3o da Prov\u00edncias; UC lidera estudo internacional sobre o combate \u00e0 doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): Litoral Centro – Comunica\u00e7\u00e3o e Imagem; Projeto PineWALL prop\u00f5e estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): Luso.eu; Projeto PineWALL prop\u00f5e estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): Mira Online; Projeto PineWALL prop\u00f5e estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): Pa\u00eds ao Minuto; Estudo: Por que h\u00e1 pinheiros que resistem ao nem\u00e1todo e outros n\u00e3o?<\/a><\/p>\n

Online News article (Portugal): Mundo Portugu\u00eas; Estudo quer apurar por que raz\u00e3o uns pinheiros resistem ao nem\u00e1todo e outros n\u00e3o.<\/a><\/p>\n

Online News article (Portugal): Not\u00edcias de Coimbra; Estudo quer apurar por que raz\u00e3o uns pinheiros resistem ao nem\u00e1todo e outros n\u00e3o.<\/a><\/p>\n

Online News article (Portugal): FCTUC-Noticias; Projeto PineWALL prop\u00f5e estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Russia): Pravda.ru; Projeto PineWALL prop\u00f5e estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): Press Point; Doen\u00e7a da murchid\u00e3o do pinheiro: projeto PineWall prop\u00f5e estrat\u00e9gia de combate.<\/a><\/p>\n

Online News article (Portugal): AGROneg\u00f3cios; Projeto PineWALL prop\u00f5e estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): Not\u00edcias do Nordeste; Projeto PineWALL prop\u00f5e estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): Agriterra; Projeto PineWALL: uma estrat\u00e9gia inovadora para combater a doen\u00e7a da murchid\u00e3o do pinheiro.<\/a><\/p>\n

Online News article (Portugal): AGROTEC; Projeto PineWALL: Estrat\u00e9gia inovadora para a doen\u00e7a da murchid\u00e3o do pinheiro. <\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Pine-related goods and activities make up a substantial share of the Portuguese forestry sector, and more generally, of the Portuguese Economy. Therefore, it is vital for this resource to be protected, especially as uncertain economic times lie ahead. The pinewood nematode (PWN), Bursaphelenchus xylophilus, is a major threat to this sector, as it causes the […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":596,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2847","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/qfm.uc.pt\/index.html\/wp-json\/wp\/v2\/pages\/2847","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/qfm.uc.pt\/index.html\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/qfm.uc.pt\/index.html\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/qfm.uc.pt\/index.html\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/qfm.uc.pt\/index.html\/wp-json\/wp\/v2\/comments?post=2847"}],"version-history":[{"count":3,"href":"https:\/\/qfm.uc.pt\/index.html\/wp-json\/wp\/v2\/pages\/2847\/revisions"}],"predecessor-version":[{"id":4846,"href":"https:\/\/qfm.uc.pt\/index.html\/wp-json\/wp\/v2\/pages\/2847\/revisions\/4846"}],"up":[{"embeddable":true,"href":"https:\/\/qfm.uc.pt\/index.html\/wp-json\/wp\/v2\/pages\/596"}],"wp:attachment":[{"href":"https:\/\/qfm.uc.pt\/index.html\/wp-json\/wp\/v2\/media?parent=2847"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}